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Well, I once had my character pour Holy water on a demon to interrogate it. And the gm at the table started squealing "That is torture and a decidedly evil act!"
My character was lawful neutral and did not see any reason why it would change his alignment. We were KILLING demons left and right and you tell me this Holy water act is going to make my character less Good? Really. Sometimes it's really how much a (insert degradatory word here) the host wants to be.
But in essence, no a level 1 would not detect outright. It is stated in the books. However this is where roleplay is intended, if it were up to me I would not have the creature be absolutely clean either. Maybe an uncomfortable feeling that could very well just be the smell the creature was giving off.
-Square