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¿If a fighter that choose "Hammer" for his highest proficiency is using a shifting rune does he lose his "Fighting Weapon Mastery"?
Shifting Rune: https://2e.aonprd.com/Equipment.aspx?ID=302
Yes.
You're very good at hammers, but if your weapon isn't shaped like a hammer that's not good for you.
I agree with Ascalaphus. It becomes a different weapon. The fact that magic is causing it to change doesn't really change that.