Simple question really, so I'm hoping the answer is equally simple.
Changelings are all female.
When using the disguise skill it is more difficult for a character to disguise themselves as a gender other than their own.
Girdle of Opposite Gender can swap a characters biological sex.
When making magic items, one of the restrictions that can be put on an item is "only usable by X gender" which works the same as racial restrictions. I don't think there are any official items that use that restriction, but it is in the custom items rules.
In the Skull and Shackles path there is an NPC who is a womanizer and easier to use diplomacy on for female characters.
Those are the ones that come up off the top of my head.
Well I'm playing around with a concept for a Kitsune who has been able to use "Realistic Likeness" to freely change shape into essentially any race and any gender for so long he has forgotten which he is. So I mean technically he would be a male but he wouldn't really be sure of it.
EDIT: Never mind, found an editor's note that rues this. :(
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One of the alien races exhibits sexual dimorphism, if I recall correctly. I believe the males and females have different stat blocks.
Correct. ^_^
Kitsune can not gender swap with their shape change.
There is a penalty to disguise skill if you are trying to disguise yourself as a different gender.
Shouldn't the Girdle of Opposite Gender be called the Girdle of Opposite Sex? I'm surprised the staff at Paizo kept the old name.
It might also come up when dealing with a couple specific creatures too, since they are more often flavor driven.
Andrazku are demons born from those that abused women, and they cannot stand working under a female. Which means a -10 to your CHA check when you summon and bargain with the things (unless you inflate their ego and basically beg them for help. Yeah, that is part of their entry- just do a bluff check with a bonus since the fools want to believe you are begging).
Admittedly, that example is just a big dumb CR 5 demon with a couple "...eh" SLAs and abilities. So it is not too big a loss, I suppose, since you can always find better beat sticks. I mostly cited it since there are all those flavor influenced rules when you summon outsiders, which made it a good example.
Overall though, gender is usually not a rules issue. You have to go looking for random side material that was written almost purely for flavor in order to find anything with solid mechanics.
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A few npcs and probably a few monsters treat you differently if you're a certain gender.
Unless stated otherwise, all characters in Golarion are supposed to be assumed bi. Pretty sure a writer for the setting said that once.
Not precisely, at least to my knowledge.
One of the Jade Regent campaign traits notes that the specified NPCs (Sandru, Ameiko, and Shalelu) are bisexual unless your GM decides otherwise. That's where the idea started, I believe.
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To answer the original question, no not really.
As shown above, there are some situations where the sex/gender/preference of the character is brought up, but it generally doesn't LIMIT actions you can take as a character.
99.9% of the time you can play your STR 20+ Barbarian as a female with zero mechanical disadvantage compared to playing a male.
That's kinda part of the charm of the system.
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Biological sex, relevant in a few cases. Gender identity, always a fluff thing.
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I have played male and female characters:
I once had a romantic swashbuckler who foolishly lost his life to the deceptive ploy of a succubus; a couple of kisses and he was undone.
I also had a female 2nd ed Psionicist back in the nineties with life discipline thing, animal affinity power, claws, natural armor, frenzied fighting, regeneration, Strength boost, sense enhancing powers: survival, tracking, some sort of stealth non weapon proficiency, two weapon fighting...
I rolled a 1 on an activation check and the rules of the power stated character was Stuck in hybrid form. Nothing like a 6'4" 200 lbs were-cheetah to scare people, NPCs were often horrified and formed mobs to hunt my character down. I found myself relieved and grateful when my fellow adventures rallied around my character and protected her. I do not know if the character's appearance being assumed to look like Brittany Diggers from Antarctic press' Gold Digger comics by Fred Perry had anything to do with all the attention my character was getting: positive and negative.
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Mechanically, not often.
When it does matter, it isn't too significant.
I would argue the Unicorn is the most relevant of the examples, though I don't think you can get one as a mount using game rules per se... (I mean as class feature/feat etc-back in 2nd edition I think there was more stuff affected by sex/gender/pick your non-offending term).
Only compelling reason to play a female character, outside of RP consideration, i would say.